基于~1H-MRS与LCModel软件检测健康青年人服用磷酸可待因后前额叶及海马代谢物变化Metabolic concentrations alternations of bilateral prefrontal lobes and hippocampus after taking codeine phosphate: quantified by ~1H-MRS and LCModel
蓝海龙;吴美联;曹震;肖叶玉;吴仁华;
摘要(Abstract):
目的通过绝对定量分析健康青年人服用磷酸可待因后双侧前额叶及海马代谢物浓度变化,为下一步研究青年人可待因慢性成瘾机制提供前期实验数据参考。材料与方法正常健康青年志愿者20名,男10名,女10名,年龄18~30岁,平均(24.9±1.9)岁,均为右利手,无神经、精神疾病病史。采用GE 1.5 T Signa HDX超导MR扫描仪,单体素1H-MRS PRESS序列,TR 3000 ms,TE 30 ms,矩阵256×128,NEX为1,感兴趣区置于双侧前额叶及双侧海马,大小2 cm×2 cm×2 cm,采集服药前及口服60 mg磷酸可待因后1.0~2 h内的实验数据,利用LCModel软件对采集的数据进行后处理及定量,用SPSS19.0配对样本t检验进行统计学分析。结果服用磷酸可待因后左前额叶脑代谢物发生改变,甘油磷酸胆碱(GPC)浓度增加0.254 mmol/L、肌醇(Ins)浓度下降0.988 mmol/L,差异有统计学意义(n=13,P<0.05;n=12,P<0.05);右前额叶、双侧海马的代谢物浓度改变无统计学差异。结论口服磷酸可待因能引起左前额叶代谢物的改变,GPC浓度上升与Ins浓度下降可能与急性用药后前膜递质释放减少有关。
关键词(KeyWords): 可待因;脑疾病;磁共振波谱学;脑代谢物,定量
基金项目(Foundation): 国家自然基金(编号:81371612);; 广东省自然科学基金(编号:S2013010013867)
作者(Author): 蓝海龙;吴美联;曹震;肖叶玉;吴仁华;
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参考文献(References):
- [1]Wei SN,Sun XJ,Zhu SY.Study progress of neuroimaging on internet addiction.Chin J Magn Reson Imaging,2010,1(5):392-396.尉胜男,孙学进,朱守艳.网络成瘾的神经影像学研究进展.磁共振成像,2010,1(5):392-396.
- [2]Liu HQ,Bai B.Progress receptor mechanism of opiate addiction.Chin J Behav Med Brain Sci,2011,20(6):571-573.刘海青,白波.阿片类药物成瘾的受体机制研究进展.中华行为医学与脑科学杂志,2011,20(6):571-573.
- [3]WU JL,LIU T,LIN Y.Absolute quantification of swine brain glutamate compounds concentration using MR spectroscopy and LCModel after nasal spraying butorphanol tartrate.Int J Med Radiol,2008,31(4):217-220.伍建林,刘婷,林艳.应用~1H-MRS和LCModel研究鼻喷酒石酸布托啡诺对猪脑代谢物的影响及谷氨酸复合物的绝对定量.国际医学放射学杂志,2008.31(4):217-220.
- [4]Damore D,Rutledge J,Pan S,et al.Handedness effects on procedural training in pediatrics.Clin Pediatr(Phila),2009,48(2):156-160.
- [5]Xu NJ,Bao L,Fan HP,et al.Morphine withdrawal increases glutamate uptake and surface expression of glutamate transporter GLT1 at hippocampal synapses.Neuroscience,2003,23(11):4775-4784.
- [6]Traudt CM,Tkac I,Ennis KM,et al.Postnatal morphine adInsnistration alters hippocampal development in rats.J Neurosci Res,2012.90(1):307-314.
- [7]Deng Y,Bu Q,Hu P,et al.~1H-nuclear magnetic resonance-based metabonoInsc analysis of brain in rhesus monkeys with morphine treatment and withdrawal intervention.J Neurosci Res,2012,90(11):2154-2162.
- [8]Gao H,Xiang Y,Sun N,et al.Metabolic changes in rat prefrontal cortex and hippocampus induced by chronic morphine treatment studied ex vivo by high resolution~1H NMR spectroscopy.Neuroche Insstry int,2007,50(2):386-394.